
The use of two-dimensional profiles in morphometric studies is prone to errors as it does not directly reflect the object numbers and size. The technique derives three-dimensional features of an object or matter ordinarily observed as a profile in two dimensions. It primarily focuses on geometrical features of objects such as number, density, length, area, and volume. It is a quantitative and comparative method that utilizes planes, lines, and points for the estimation of three-dimensional parameters in morphological studies. Certainly, the application of stereological techniques provides a pragmatic approach in histological analyses and has driven most of advances in morphometric and biological image analyses. Primarily these are practical techniques for extracting quantitative information about a three-dimensional material from measurements made on two-dimensional planar sections of the material. Stereology refers to three-dimensional interpretation of two-dimensional cross sections of materials or tissues. Quantitative investigation of images taken from light microscopy observation is one of the pillars of biological and biomedical investigation and is achieved through stereological methods. Given the growing importance of stereology in biomedical research, it is necessary to promote its application among scholars. The highly cited article had a total of 2,054 citations with an average of over 82 citations per year. Nyengaard was the most prolific author who contributed at least 125 publications. The most productive country was USA with at least 1663 (23.10%) publications and Aarhus Universitet tops the list of institutions with at least 306 publications.

The Journal of Microscopy tops the list of journals with at least 205 articles. A great majority (5,332 93.02%) of the publications retrieved from SCOPUS were journal articles in various biomedical fields. The relative growth rate (RGR) of publications decreased from 1967 (0.69) to 2016 (0.03) whereas the doubling time (DT) increased from 1.00 to 20.56 in the same period.

Half (2,858 49.87%) of these publications were produced in the last 12 years from 2005 to 2016. At least 5,732 publications employing stereology as analytical tool were produced in a period of 50 years between 19. Data for this study were retrieved from the SCOPUS database. Stereology has gained wide application resulting into design-based stereological methods. A scientometric study was conducted to analyze global research trends in application of stereology in biomedical research. Stereology is a quantitative and comparative method that utilizes planes, lines, and points for the estimation of three-dimensional parameters in morphological studies.
